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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
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wulidanisa@berkalahayati.org
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Jalan Surakarta No. 5 Malang, Indonesia
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INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 15 Documents
Search results for , issue "Vol 10 No 1 (2004): December 2004" : 15 Documents clear
INDUKSI 2-METHOXYETHANOL PADA MASA PRENATAL SEBAGAI PENYEBAB KELAINAN OTAK PADA MENCIT Win Darmanto; Eko Prihiyantoro; Ria Harmonis
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/370

Abstract

2-Methoxyethanol (2-ME) and glycol esters are widely used as a solvents in the industry and plasticizers, and have becomes ubiquitous environmental pollutants. This study were designed to evaluate the malformation of brain development as an impact of 2-ME. Four groups of pregnant mice were injected to 2-ME dose 10 mmol/kg body weight at 7, 13, 15, and 17 days gestation respectively. Control group were injected with sterile water. At 18 days of gestation samples in groups 7 and 13 days gestation were sacrificed. Brain were remove and weight, morfologically and histologically were examined. Samples were divided into five groups, i.e. control group, 7th, 13rd, 15th and 17th days of gestation groups. Samples in 7th and 15th days of gestation groups killed in 18th days of gestation to observed fetal brain defect and decreased of fetal brain weight but some samples to allow delivery of fetuses. Furthermore, the fetuses killed on 0, 5, 10, and 20 days after birth, to take their brain. The result of these study showed that there are decreasing total weight of fetal brains and there are cerebral malformation as impact of 2-ME exposure.
EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus) Alifah Hayati; Binti Yunaida; I.B. Rai Pidada; Win Darmanto; Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/371

Abstract

This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.
KAJIAN PENDAHULUAN UJI TOKSISITAS EKSTRAK AIR MISELIA DAN TUBUH BUAH JAMUR SHIITAKE (Lentinus edodes) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST) Noor Erma NS; Tri Sundari; Arie Ika Susanty; Dwi Riani Oktavia Palupi; Isnaeni; Sukardiman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/372

Abstract

Shiitake mushroom (Lentinus edodes) is one of the wood mushroom types that can be consumed as a food as well as for a medical purpose. Lentinan, a polysaccharide contained in shiitake, is well known for its use on cancer medication. Mycelium of Shiitake mushroom contains lentinan the same as other part of the mushroom like fruity body. Toxicity of the lentinan in mycelium compare to the fruity body has been first conducted by using Brine Shrimp Lethality Test (BST). Using Potato Dextrose Broth media with the growth rate of 3.88% did mycelium multiplications. Probit analysis showed that the toxicity of the mushroom’s cap, stem, and mycelium of Shiitake mushrooms is LC50 = 648.76507 mg/ml LC50 = 489.39444 mg/ml, and LC50 = 481.16941 mg/ml respectively.
BIOAKUMULASI LOGAM BERAT Cu OLEH Bacillus sp Riesta Primaharinastiti; A. Toto Poernomo; Noor Erma S.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/373

Abstract

The research was conducted to investigate the ability of Bacillus sp in accumulating Cu and how much it can be acumulated. The medium used to growth the bacterium was Nutrient Broth and Atomic Absorption Spectrophotometry methods was used to assay the Cu, both in the cells and medium. The result of this study showed that Bacillus sp incubated in the Nutrient Broth medium containing 10 ppm of Cu, with continuous stirring in the room temperature was able to reduce Cu in the medium 8.912–12.623% and accumulate Cu in the cell 0.1149–0.1400 %/mg cells. Based on this result, it is necessary to develop more studies to find out what factors that influence the accumulation process and to optimize the bioprocess.
BIOTRANSFORMASI ADIPONITRIL OLEH Bacillus licheniformis BA2 Ahmad Thontowi; Eko W. Pamuji; Bambang Sunarko
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/374

Abstract

Adipic acid represents one of the especial materials which used for the synthesis of nylon 6,6,- is a very important material results from polyamide industry. Adiponitrile biotransformation become adipic acid represent an alternative synthesis besides chemically. The purpose of this research was to determine optimum conditions for Bacillus licheniformis BA2 growth for adiponitrile degradation, and also know its pattern. The obtained information, to be expected can be used as reference for scaling up of adipic acid production. B. licheniformis BA2 was able to utilize acetonitrile and adiponitrile as the sole source of carbon and nitrogen. The growth on adiponitrile 120 mM mixture with acetonitrile 30 mM gave higher growth rate and biomass yield than growth on another subsrates. B. licheniformis BA2 have lag phase during 68 hours, logaritmic phase passed by during 104 hours, while stasioner phase just reached by after 172 hours. High-performance liquid chromatography of adiponitrile degradation by crude bacterial revealed a decrease in adiponitrile with the sequential formation of adipamide and adipic acid. Ammonia was also detected by colorimetric procedures. As for adipic acid rendemen at 420 minutes equal to 19.35%.
CHARACTERISTICS OF THE PEAT SOIL Bacilllus sp. Iman Hidayat
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/375

Abstract

Twenty two strains of Bacillus species have been isolated from the peat soil of Tanjung Puting, Central Kalimantan. All strains devided into seventeen groups which characterized using morphology and biochemical tests. All strains have been tested for lipase, amylase, protease, cellulase, and phosphatase activity. Thirteen Bacillus isolates tested gave positif reaction for lipase activity. Bacillus AR 018 was the best isolate for amylase activity, Bacillus AR 001 and Bacillus AR 002 for protease activity, Bacillus AR 009 for cellulase activity. Only one isolate shows positive reaction for phosphatase activity, notably Bacillus AR 007.
IDENTIFIKASI BERAT, DIAMETER, DAN TEBAL DAGING BUAH MELON (Cucumis melo, L.) KULTIVAR ACTION 434 TETRAPLOID AKIBAT PERLAKUAN KOLKISIN Y. Ulung Anggraito
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/376

Abstract

Indonesian farmers are very dependence on certificated seed from another countries. In the other side the natural resources and men powers very abundance. For these reason it is properly developed the research in agriculture sector, especially on plants breeding. It can be hoped that in the future the dependence on certificated seed from another countries can be minimized. The objective of this research were: (1) to find out the concentration and dipping period which is effective to induce polyploid in musk melon plant, (2) identify the weight, diameter, dan flesh thickness of tetraploid musk melon as result of colchicines treatment. The sample of this research was Action 434 musk melon cultivar, product of Chia-Thai Seed, Thailand. The number of sample was 480 plants, which plants on field randomly. There were four colchicines concentration as an independent variable: 0.0%, 0.05%, 0.10% and 0.2%. The dipping period were 12, 16, 20, and 24 hours for each concentration respectively. Completely Random Design was used in three replications. Data measurement were analyzed with Two Way ANOVA, DMRT, and LSD. From this research can be concluded that: (1) 0.2 % colchicine is the most effective concentration to induce polyploid on musk melon, with dipping period effective varied from 16–24 hours, (2) there are changes in weight, diameter, and flesh thickness characters, with the increased tendency of each character in definite norm.
8-HIDROKSI-DEOKSIGUANOSIN SEBAGAI SALAH SATU INDIKATOR INFERTILITAS PRIA Sudjarwo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/377

Abstract

DNA in human’s sperm is located in nucleus (nDNA) and mitochondria (mtDNA). DNA oxidation is indicated from high concentration of 8-OH-dG. It is very important to identify factors that can cause genetic dysfunction risk responsible for genetic changes. 8-OH-dG compound are a promutagenic of DNA dysfunction of deoxyguanosine oxidation by oxygen radical. ROS is an oxidation, which in high concentration can oxidize DNA. Guanine is a nucleotide, which prone to oxidation, with 8-OH-dG as the end product. Detection of 8-OH-dG can be done by HPLC method. These results indicated that there were significant differences (p < 0.01) in detection of 8-OH-dG in sperm, between normospermia with asthenospermia and normospermia with oligospermia. Correlation (r) of 8-OH-dG with motile spermatozoa is – 0.7111. Immotile spermatozoa caused by oxidation or DNA damage are shown by high concentration of 8-OH-dG. In normal physical condition, sperm produces oxidant. High oxidant is toxic to sperm, which influence their motility. Sperm’s motility is one of factors causing male infertility.
PERBANDINGAN PENINGKATAN BERAT BADAN ANAK MENCIT YANG DIINDUKSI OLEH PEMBERIAN INFUS DAUN PEPAYA DAN DAUN KATU IB. Rai Pidada
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/418

Abstract

A comparative study of increasing on body weight of mice ,s offsprings caused by leaf of papaya (Carica pepaya L.) and katu (Sauropus androgynus (L). Merr) were carried out under laboratory conditions. The study was designed using totally 50 mice post partum (PP) which devided into five groups of treatment consistent 10 females mice post partum of each group. The groups were: The control group, group gave orally in 0.5 ml aquadest, 10%/0.5 ml, and 20%/0.5 ml leaf infuse of papaya and 10%/0.5 ml and 20%/0.5 ml leaf infuse of katu respectively. The treatment were started on day 3rd to 16th of lactation period. The sample were observed on day 4th, 6th , 8th, 10th, 12nd, 14th, and 16th of lactation period. The data were analyzed by ANOVA and LSD test (α = 0.05). The result of study showed that the increasing of body weight of offsprings were significantly different amongs of five groups. Between the control groups and katu groups was not occurred significantly different. The conclusion of this study is papaya groups more right than katu groups to induce an increasing on body weight of mice,s offsprings.
PENGARUH INFUS RIMPANG TEMULAWAK (Curcuma xanthorrhiza) TERHADAP KADAR HEMOGLOBIN DAN JUMLAH ERITROSIT TIKUS PUTIH YANG DIBERI LARUTAN TIMBAL NITRAT [(PbNO3)2] Sugiharto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/419

Abstract

The purpose of this experiment was to study the effect of turmeric (Curcuma xanthorrhiza) rhizome infuse to hemoglobin concentration and number of erythrocyte of [(PbNO3)2] treated rats. Pb was presumed to be link at sulphyhydril groups that may caused an inhibition to several enzymatic process, such as d-ALAD and heme sintetase which is caused the inhibits of Hb synthesis and erythropoesis. Twenty female rats was used in this experiment. They were divided into five groups, i.e. (A) control (treated with 1 ml aquadest); (B) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml aquadest; (C) treated with 1/2 ml of 12 ppm lead solution and 1/2 ml of 20% turmeric rhizome infuse; (D) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml aquadest; (E) treated with 1/2 ml of 50 ppm lead solution and 1/2 ml of 20% turmeric rhizome infuse. The treatment was given orally every day (30 days) using a modified syringe. After 30 days of treatment, the blood sample were taken about 2 ml by heart puncture. The Hb concentration was determined using by Cyanmethemoglobin method and number of erythrocyte was counting on Haemocytometer Improved Neubauer. Data were analyzed by Anova and LSD test (α = 0.05). The results of this study shows that 20% of turmeric rhizome infuse statistically has a significant effect (P < 0.05) to increasing of hemoglobin concentration but hasn’t significant to prevent the number of erythrocyte decreasing of [(PbNO3)2] treated.

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